Sftpc creert2

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August undefined, 2024

WebAlveolar type II (AT2) cell dysfunction contributes to a number of significant human pathologies including respiratory distress syndrome, lung adenocarcinoma, and … Web28 May 2024 · The total AT2 population (Sftpc Pos Tom Pos cells) was isolated from lungs of 6-8 weeks old Sftpc CreERT2/+; tdTomato flox/flox mice. For the single-cell preparation, lungs were inflated intratracheally with 3 mL dispase (5 U/mL; BD Biosciences #354235) and for further digestion, each lung was incubated in 3 mL dispase solution (5 U/mL) at room …

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Web12 Jul 2024 · SFTPC creERT2 mice were generated as previously described . To generate the Gli2 creERT2-tdT mouse, a CreERT2-2A-tdTomato expression cassette was inserted … Web7 Oct 2024 · To assess the relative contributions of AT2 and AT1 cells to tissue regeneration after hyperoxic injury, we lineage traced AT2 and AT1 cells using the SftpcCreERT2;R26REYFP and HopxCreERT2;R26REYFP alleles, respectively. rolling on the river flushed away

Sftpc-IRES-Cre

WebRock et al. employed the surfactant protein C (Sftpc)-CreERT2 driver to label type 2 alveolar epithelial cells (ACE2) with a red fluorescent protein (RFP) in an intratracheal bleomycin model. They demonstrated that ACE2 cells do not contribute to fibroblasts that accumulate in fibrotic lung lesions. Similar conclusions were reached using the ... Web丁香通为您提供Plch2Flox商品详情介绍：价格：询价，货号：NM-CKO-2112365，品牌：南模生物，产地：中国上海，详见丁香通Plch2Flox商品详情页； Web15 Jun 2024 · The total AT2 population (Sftpc Pos Tom Pos cells) was isolated from lungs of 6-8 weeks old Sftpc CreERT2/+; tdTomato flox/flox mice. For the single-cell preparation, lungs were inflated intratracheally with 3 mL dispase (5 U/mL; BD Biosciences #354235) and for further digestion, each lung was incubated in 3 mL dispase solution (5 U/mL) at room … rolling one\u0027s eyes

Early lineage specification defines alveolar epithelial ontogeny in …

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Web10 May 2024 · Citation: Ahmadvand, N.; Carraro, G.; Jones, M.R.; Shalashova, I.; Noori, A.; Wilhelm, J.; Baal, N.; Khosravi, F.; Chen, C.; Zhang, J.-S.; et al. Cell-Surface ... Web6 Feb 2024 · However, tamoxifen-treated Sftpc CreERT2; hACE2 fl/y males infected with SARS-CoV-2 virus exhibited levels of viral nucleocapsid protein and viral RNA in the lung indistinguishable from those in hACE2 fl/y animals (Fig 3B and 3C). These findings suggested that cell types other than AT2 cells can support SARS-CoV-2 infection of the lung. rolling online diceWebMutations in human and/or mouse homologs are associated with this disease. Synonyms: lung neoplasm rolling on wheels

"WebCx3cr1 CreERT2 and Sall1 CreERT2 mice were crossed to two reporter strains: R26-CAG-ZsGreen and R26-YFP (Fig. 1A). In R26-CAG-ZsGreen mice, the reporter construct consists of the ZsGreen sequence placed downstream of three floxed SV40 polyA sequences (0.9 kb) that block transcription from the strong artificial CAG promotor 12. " - Sftpc creert2

Sftpc creert2

Metastatic-niche labelling reveals parenchymal cells with stem …

WebSftpcCreERT2/+;tdTomatoflox/flox mice were used for the labelling of AT2 cells and labelled subpopulations were analysed by flow cytometry, quantitative PCR, assay for transposase-accessible chromatin using sequencing (ATAC-seq), gene arrays, pneumonectomy and culture of precision-cut lung slices. Web4 Nov 2024 · Sftpc CreERT2/+;tdTomato flox/flox mice were used for the labelling of AT2 cells and labelled subpopulations were analysed by flow cytometry, quantitative PCR, …

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Web南模生物. Language: 中文 . English; 日本語; 한국어; 账户设置; 退出 Web29 Nov 2024 · D, E Quantification of samples from the Sftpc CreERT2 driver line (D) and from the Hopx CreERT2 driver line (E). In the Sftpc-CreERT2 driver line (D), (a) the percentage of HOPX pos lineage-labeled RFP pos cells over the total number of RFP pos cells increases in experimental versus control lungs (33.25% ± 2.08% vs. 19.99% ± 0.89%); …

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WebTamoxifen诱导Cre-ERT2小鼠使用指南. Cre-ERT2在无Tamoxifen诱导的情况下，在细胞质内处于无活性状态；当Tamoxifen诱导后，Tamoxifen的代谢产物4-OHT（雌激素类似物） … Web008179 B6.129S4- Kras tm4Tyj /J This Kras LSL-G12D strain carries a Lox-Stop-Lox (LSL) sequence followed by the K-ras G12D point mutation allele commonly associated with …

WebUsing the Sftpc CreERT2; tdTomato lineage-traced mice, we recently reported a novel AT2-IAAP subpopulation characterized by low Sftpc expression but that is enriched for PD-L1 expression . IAAP cells, which represent quiescent and immature epithelial progenitors, undergo activation and expansion following PNX injury .

Web2 Mar 2024 · The Sftpc CreERT2/+ mice (on mixed Balbc/C567 background) were a kind gift from E.E. Morrissey (University of Pennsylvania; originally made in Dr. H. Chapman’s group [Chapman et al., 2011] and has been used widely in the field to target AT2 cells). The animals were back crossed for four to five generations onto a C57 background. rolling on the river who madeWeb20 Jan 2024 · Quantitatively, AT2 cells from Sftpc CreERT2; Rosa26 miR-302 lungs exhibited increased mitochondrial area and decreased cristae density per mitochondrion, compared with Sftpc CreERT2 controls . These results suggest that decreased AT2-to-AT1 cell differentiation in Sftpc CreERT2 ; Rosa26 miR-302 lungs may be due to miR-302 inhibiting … rolling online gameWeb25 Sep 2012 · A number of tissue-specific Cre-ER T2 mouse models have been reported [7], [8], [9]. In this study, we developed a novel SPC-Cre-ER T2 mouse model which can be applied for gene knock-out in mouse alveolar epithelium in a temporally controlled fashion. We tested this model in both ROSA26R and TSC1 fx/fx transgenic mice. rolling open earth\u0027s deepest forearc basinWeb10 Jan 2024 · Lungs from WT, Sftpc CreERT2 R26R EYFP, and Axin2 CreERT2–TdTomato mice were processed into single-cell suspensions as indicated above. WT AT2 cells: AT2 … rolling open backed shelvesWeb28 Mar 2024 · ( C) SftpcCreERT2/+RosatdTomato/+ lung after dosing with tamoxifen for 5 d and then s.c. dosing with bleomycin for 1 wk. Arrowheads point to the nuclei of AT2-derived AT1 cells. The differentiation of AT2 cells into the AT1 cells that mediate pulmonary gas exchange is critical for replacing damaged AT1 cells after lung injury. rolling on wall textureWeb7 Oct 2024 · To assess the relative contributions of AT2 and AT1 cells to tissue regeneration after hyperoxic injury, we lineage traced AT2 and AT1 cells using the … rolling open cartWebMouse Lines Sftpc Creert2, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article … rolling operating cash flow