WebPerCP-Cy5.5–labelled antibodies can be used with FITC- and R-PE–labelled reagents in single-laser flow cytometers with no significant spectral overlap of PerCP-Cy5.5, FITC, and R-PE fluorescence. PerCP-Cy5.5 is optimized for use with a single argon ion laser emitting 488-nm light. Because of the broad absorption spectrum of the tandem ... WebCy5 Labeling. Cy5/ Cyanine 5 are popular fluorescent dyes compatible with various detection techniques and standard filters. This class includes far-red fluorescence …

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WebFlow cytometric analysis of CD56 expression on human peripheral blood lymphocytes. Whole blood was stained with PerCP-Cy™5.5 Mouse Anti-Human CD56 antibody (Cat. No. 560842; solid line histogram) or with a PerCP-Cy™5.5 Mouse IgG1, κ Isotype Control (Cat. No. 550795; dashed line histogram). The erythrocytes were lysed with BD PharmLyse ... WebCy5.5-labeled TCL-SPION had the highest fluorescence density in the lung at 0.5 h post-injection and decreased rapidly over time. Fluorescence density in liver and spleen was … frederick ian eyewear boulder

Cyanine 5.5 monosuccinimidyl ester [equivalent to Cy5.5® NHS est…

WebWhole blood was stained with PerCP-Cy™5.5 Mouse Anti-Human CD15 (Cat. No. 560828; solid line histogram) or with a PerCP-Cy™5.5 Mouse IgM, κ Isotype Control (Cat. No. 560857; dashed line histogram). The erythrocytes were lysed with Lysing Buffer (Cat. No. 555899). The fluorescence histograms were derived from gated events with the forward ... WebJul 20, 2024 · Answer. Cy5 and Cy5.5 are both cyanine dyes with several differences between them. Used in Fluorescence Resonance Energy Transfer (FRET), Fluorescence Polarization (FP), and Time Resolved- Fluorescence Resonance Energy Transfer (TR-FRET). Used to label DNA oligos for use as hybridization probes in other applications … In microarray experiments DNA or RNA is labeled with either Cy3 or Cy5 that has been synthesized to carry an N-hydroxysuccinimidyl ester (NHS-ester) reactive group. Since NHS-esters react readily only with aliphatic amine groups, which nucleic acids lack, nucleotides have to be modified with aminoallyl groups. See more Cyanines, also referred to as tetramethylindo(di)-carbocyanines are a synthetic dye family belonging to the polymethine group. Although the name derives etymologically from terms for shades of See more Cyanines were first synthesized over a century ago. They were originally used, and still are, to increase the sensitivity range of photographic emulsions, i.e., to increase the range of See more The word cyanin is from the English word "cyan", which conventionally means a shade of blue-green (close to "aqua") and is derived from the Greek κυάνεος/κυανοῦς kyaneos/kyanous … See more Cyanines have been classified in many ways: • Streptocyanines or open chain cyanines: R2N … See more Because they yield brighter and more stable fluorescence, cyanines can advantageously replace conventional dyes such as fluorescein and rhodamines. • Cy3 and Cy5 are the most popular, used typically combined for 2 colors detection. Cy3 fluoresces … See more frederick hypnosis