WebMay 18, 2024 · RDB04383. Resource name. CSII-CMV-MCS-IRES2-Venus. Clone info. Backbone vector plasmid of lentivirus construct driven by CMV promoter with Venus fluorescent protein marker. Comment. Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is … WebNov 14, 2009 · CMV IE (human cytomegalovirus immediate early promoter): 1–589 • MCS (multiple cloning site): 591–665 • IRES2 (encephalomyocarditis virus internal ribosome entry site): 666–1250 • DsRed-Express2 (Discosoma sp. red fluorescent protein variant) Start codon (ATG): 1254–1256; Stop codon: 1929–1931 • SV40 early polyA +
Robust Enhancement of Lentivirus Production by Promoter Activation
WebCSII-CMV-MCS CMV promoter, Multiple cloning site: RDB04378: CSII-EF-MCS EF-1α promoter, Multiple cloning site: RDB05963: CS-CA-MCS CAG promoter, Multiple cloning … WebFeb 6, 2024 · The images of the specimens were obtained using an Aperio CS2 scanner (Leica Biosystems, Wetzlar, Germany). ... These cDNAs, along with a FLAG Tag, were ligated into CSII-CMV-MCS-IRES2-Bsd (RIKEN ... chitharal rock temple
Does pIRES2 express more EGFP than the mRNA before the IRES
WebInside US: +1 (877) 432-9908 Bulletin No. CSMSTRV2-E Outside US: +1 (717) 767-6511 Drawing No. LP0641 www.redlion.net Released 2024-10-13 -1-MODEL CSMSTRV2 ‐ … WebCSII-CMV-MCS-IRES2-Bsd-VMO1 or CSII-CMVMCS-IRES2-Bsd-luciferase plasmid was transfected into HEK293T cells using the Lentivirus High Titer Packaging Mix (Takara Bio Inc., Shiga, Japan) for lentivirus produc-tion. After 6 h of transfection, the cells were washed with PBS and cultured in serum-free DMEM for 48 h. After WebJan 21, 2024 · Human TMPRSS2 and ACE2 genes were cloned into the self-inactivating lentiviral vector plasmids, CSII-CMV-MCS-IRES2-Bsd (RIKEN BRC) and pLVSIN-CMV Pur (Takara Bio), respectively. The resulting constructs were named CSII-CMV-TMPRSS2-IRES2-Bsd and pLVSIN-CMV-ACE2-Pur. For lentiviral vector preparation, 293T cells … grappling hold crossword clue